Dr Chris O'Brien
Research Fellow
Queensland Alliance for Agriculture and Food Innovation
+61 7 334 66519
Researcher biography
Chris O'Brien is a research fellow at QAAFI leading the avocado tissue culture, gene editing and cryopreservation program. He is a global expert in optimising tissue culture and in vitro technologies for recalcitrant crops. His pioneering work on propagation and cryopreservation of avocado has contributed to global licensees for technology uptake. Chris is passionate to deliver world-first technology platforms for horticultural crop improvement. This innovative tech-development will address critical challenges of traditional breeding, enabling rapid development of future-smart cultivars.
Book Chapter
Hiti-Bandaralage, Jayeni, Hayward, Alice, O’Brien, Chris, Gleeson, Madeleine, Nak, William and Mitter, Neena (2020). Advances in avocado propagation for the sustainable supply of planting materials. Achieving sustainable cultivation of tropical fruits. (pp. 215-237) Cambridge, United Kingdom: Burleigh Dodds Science Publishing. doi: 10.19103/as.2019.0054.19
Journal Articles
Bao, Jingyin, O’Donohue, Billy, Sommerville, Karen D., Mitter, Neena, O’Brien, Chris and Hayward, Alice (2024). Tissue culture innovations for propagation and conservation of Myrteae—a globally important Myrtaceae tribe. Plants, 13 (16) 2244, 2244. doi: 10.3390/plants13162244
O'Donohue, Billy, Hiti-Bandaralage, Jayeni, Gleeson, Madeleine, O'Brien, Chris, Harvey, Maggie-Anne, van der Ent, Antony, Pinto Irish, Katherine, Mitter, Neena and Hayward, Alice (2022). Tissue culture tools for selenium hyperaccumulator Neptunia amplexicaulis for development in phytoextraction. Natural Products and Bioprospecting, 12 (1) 28, 1-9. doi: 10.1007/s13659-022-00351-2
O’Brien, Chris, Hiti-Bandaralage, Jayeni, Folgado, Raquel, Hayward, Alice, Lahmeyer, Sean, Folsom, Jim and Mitter, Neena (2021). Cryopreservation of woody crops: The avocado case. Plants, 10 (5) 934, 934. doi: 10.3390/plants10050934
O'Brien, C., Hiti-Bandaralage, J. C. A., Folgado, R., Lahmeyer, S., Hayward, A., Folsom, J. and Mitter, N. (2020). First report on cryopreservation of mature shoot tips of two avocado (Persea americana Mill.) rootstocks. Plant Cell Tissue and Organ Culture, 144 (1), 103-113. doi: 10.1007/s11240-020-01861-y
O’Brien, C., Hiti-Bandaralage, J., Folgado, R., Lahmeyer, S., Hayward, A., Folsom, J. and Mitter, N. (2020). A method to increase regrowth of vitrified shoot tips of avocado (Persea americana Mill.): First critical step in developing a cryopreservation protocol. Scientia Horticulturae, 266 109305, 109305. doi: 10.1016/j.scienta.2020.109305
Hiti-Bandaralage, Jayeni, Hayward, Alice, O’Brien, Chris, Ahsan, Umair, Gleeson, Madeline, Xue, Yuxin and Mitter, Neena (2020). Phase change related microRNA profiles in the plant regeneration process of avocado through shoot-tip culture. Annals of Advanced Agricultural Sciences, 4 (2). doi: 10.22606/as.2020.42001
O'Brien, C., Constantin, M., Walia, A., Yiing, J. L. Y. and Mitter, N. (2016). Cryopreservation of somatic embryos for avocado germplasm conservation. Scientia Horticulturae, 211, 328-335. doi: 10.1016/j.scienta.2016.09.008
Encina, Carlos López, Parisi, Ann, O'Brien, Chris and Mitter, Neena (2014). Enhancing somatic embryogenesis in avocado (Persea americana Mill.) using a two-step culture system and including glutamine in the culture medium. Scientia Horticulturae, 165, 44-50. doi: 10.1016/j.scienta.2013.10.019
Hacker, Andrew, Puachuay, Meena, Max Shelton, H., Dalzell, Scott, Al Dabbagh, Haider, Jewell, Margaret, van Tran, Thinh, Ngo, The, Zhou, Yi, Halliday, Michael, Giles, Hayley, Loch, Don S., Godwin, Ian D., Fukai, Shu, O'Brien, Chris, Pearce, Will, Anderson, William F. and Lambrides, Christopher J. (2013). Potential of Australian bermudagrasses (Cynodon spp.) for pasture in subtropical Australia. Tropical Grasslands-Forrajes Tropicales, 1 (1), 81-83. doi: 10.17138/TGFT(1)81-83
O'Brien, C. M. and Drew, R. A. (2009). Potential for using Vasconcellea parviflora as a bridging species in intergeneric hybridisation between V. pubescens and Carica papaya. Australian Journal of Botany, 57 (7), 592-601. doi: 10.1071/bt09111
Drew, R.A., Siar, S.V., O'Brien, C.M. and Sajise, A.G.C. (2006). Progress in backcrossing between Carica papaya x Vasconcellea quercifolia intergeneric hybrids and C. papaya. Australian Journal of Experimental Agriculture, 46 (3), 419-424. doi: 10.1071/ea04248
Drew, R.A., Siar, S.V., O'Brien, C.M., Magdalita, P.M. and Sajise, A.G.C. (2006). Breeding for Papaya ringspot virus resistance in Carica papaya via hybridisation with Vasconcellea quercifolia. Australian Journal of Experimental Agriculture, 46 (3), 413-418. doi: 10.1071/ea04247
Van Droogenbroeck, B., Maertens, I., Haegeman, A., Kyndt, T., O'Brien, C., Drew, R.A. and Gheysen, G. (2005). Maternal inheritance of cytoplasmic organelles in intergeneric hybrids of Carica papaya L. and Vasconcellea spp. (Caricaceae Dumort., Brassicales). Euphytica, 143 (1-2), 161-168. doi: 10.1007/s10681-005-3156-0
Conference Papers
Li, Xueying, O’Brien, Chris, Sommerville, Karen, Mitter, Neena and Hayward, Alice (2023). Innovative cryopreservation technology for Australian macadamia. 60th Annual Meeting of the Society for Cryobiology CRYO2023, Minneapolis, MN United States, 25-27 July 2023. Maryland Heights, MO United States: Academic Press. doi: 10.1016/j.cryobiol.2023.104759
Bao, Jingyin, O’Brien, Chris, Sommerville, Karen, Mitter, Neena and Hayward, Alice (2023). Incorporating cold pretreatment in cryopreserving shoot tips of sweet myrtle (Gossia fragrantissima). 60th Annual Meeting of the Society for Cryobiology CRYO2023, Minneapolis, MN United States, 25-27 July 2023. Maryland Heights, MO United States: Academic Press. doi: 10.1016/j.cryobiol.2023.104742
Bao, Jingyin, O’Brien, Chris, Sommerville, Karen, Mitter, Neena and Hayward, Alice (2022). Cold pretreatment for cryopreservation of an endangered Australian native plant species Gossia fragrantissima. CRYO2022 Joint Meeting of SLTB and Society for Cryobiology, Dublin, Ireland, 19-22 July 2022. Maryland Heights, MO United States: Academic Press. doi: 10.1016/j.cryobiol.2022.11.045
Li, Xueying, O’brien, Chris, Sommerville, Karen, Mitter, Neena and Hayward, Alice (2022). Innovative cryopreservation technology for Australian macadamia. CRYO2022 59th Annual Meeting of the Society for Cryobiology, Dublin, Ireland, 19-22 July 2022. Maryland Heights, MO United States: Academic Press. doi: 10.1016/j.cryobiol.2022.11.198
O’Brien, C., Hiti-Bandaralage, J.C.A., Folgado, R., Lahmeyer, S., Hayward, A. and Mitter, N. (2020). Developing a cryopreservation protocol for avocado (Persea americana Mill.) apical shoot tips using different antioxidants. International Horticultural Congress, Istanbul, Turkey, 12-16 August 2018. Leuven, Belgium: International Society for Horticultural Science. doi: 10.17660/actahortic.2020.1285.3
Hiti Bandaralage, J. C. A., Hayward, A., O'Brien, C., Beveridge, C. and Mitter, N. (2019). Avocado micropropagation: growth in leaps and bounds for a clonal rootstock propagation solution. Fifth International Conference of the IUFRO Working Party Unit 2.09.02 “Somatic embryogenesis and other vegetative propagation technologies”, Coimbra, Portugal, 10 - 15 September 2018. Coimbra, Rua Bernardino: João Duarte, Unipessoal.
O'Brien, Chris, Bandaralage, Jayeni Hiti, Folgado, Raquel, Lahmeyer, Sean, Hayward, Alice and Mitter, Neena (2019). The Use Of Sucrose Pre-Culture For Cryopreservation Of Avocado (Persea Americana Mill.) Cultivar ‘Velvick’. 56th Annual Meeting of the Society for Cryobiology CRYO 2019, San Diego, CA United States, 22-25 July 2019. Maryland Heights, MO United States: Academic Press. doi: 10.1016/j.cryobiol.2019.10.056
Hiti Bandaralage, J. C. A., Hayward, A., O’Brien, C., Beveridge, C. and Mitter, N. (2018). Acclimatization of micropropagated mature avocado. VII International Symposium on Production and Establishment of Micropropagated Plants, Lavras, Minas Gerais, Brazil, 24-28 April 2017. Leuven, Belgium: International Society for Horticultural Science. doi: 10.17660/ActaHortic.2018.1224.3
Hiti Bandaralage, J. C.A., Hayward, A., O’Brien, C. and Mitter, N. (2018). Temporal profile of juvenility-associated microRNAs during tissue culture of avocado. International Symposia on Tropical and Temperate Horticulture - ISTTH2016, Cairns, QLD, Australia, 20-25 November 2016. Leuven, Belgium: International Society for Horticultural Science. doi: 10.17660/ActaHortic.2018.1205.97
O’Brien, Chris, Hiti Bandaralage, Jayeni C. A., Folgado, Raquel, Lahmeyer, Sean, Hayward, Alice and Mitter, Neena (2018). Developing a cryopreservation protocol for avocado (Persea Americana Mill.) shoot tips. CRYO2018, Madrid, Spain, 10-13 July 2018. Maryland Heights, MO United States: Elsevier. doi: 10.1016/j.cryobiol.2018.10.199
Hiti Bandalarage, Jayeni , Hayward, Alice , O'Brien, Christopher and Mitter, Neena (2017). Micropropagation: The Future of Clonal Propagation of Avocado. International Tropical Agriculture Conference, Brisbane, QLD, Australia, 20-22 November 2017. Brisbane, QLD, Australia: TropAg.
O'Brien, C., Parisi, A., Yuan, J. Lim Yiing, Constantin, M. and Mitter, N. (2016). Cryopreservation of avocado (Persea americana Mill.) using somatic embryos. XXIX International Horticultural Congress on Horticulture: Sustaining Lives, Livelihoods and Landscapes (IHC2014): International Symposium on Micropropagation and In Vitro Techniques, Brisbane, QLD., Australia, 17-22 August 2014. Leuven, Belgium: International Society for Horticultural Science. doi: 10.17660/ActaHortic.2016.1113.39
Hiti Bandaralage, J., Hayward, A., O'Brien, C. and Mitter, N. (2015). Gibberellin and cytokinin in synergy for a rapid nodal multiplication system of avocado. VIII World Avocado Congress, Lima, Peru, 13- 18 September 2015. Lima Peru: World Avocado Congress.
Mohd Rodzi, A., Hayward, A., Hiti Bandaralage, J., O'Brien, C., Gleeson, M. and Mitter, N. (2015). Factors affecting avocado shoot health in culture. VIII World Avocado Congress, Lima, Peru, 13- 18 September 2015. Lima Peru: World Avocado Congress.
Hacker, Andrew, Puachuay, Meena, Shelton, H. Max, Dalzell, Scott, Al Dabbagh, Haider, Jewell, Margaret, Tran, Thinh Van, Ngo, The, Zhou, Yi, Halliday, Michael, Giles, Hayley, Loch, Don S., Godwin, Ian D., Fukai, Shu, O'Brien, Chris, Pearce, Will, Anderson, William F. and Lambrides, Christopher J. (2013). Potential of Australian bermudagrasses (Cynodon spp.) for pasture in subtropical Australia. IGC2013: 22nd International Grassland Congress 2013, Sydney, NSW, Australia, 15-19 September, 2013. Santiago de Cali, Colombia: Centro Internacional de Agricultura Tropical.
Lambrides, Christopher J., Zhou, Yi, Fukai, Shu, Jewell, Margaret, Loch, Don, Godwin, Ian, Holton, Tim, Innes, David, Frere, Celine, Hanna, Wayne, Ye, Changrong, Van Tran, Thinh, Le, Thu, Cao, Ninh, Al Dabbagh, Haider, O'Brien, Chris, Roche, Matt, Fullelove, Garry, Ballard, Emma, Kearns, Ryan, Pearce, Will, Hacker, Andrew, Puachuay, Meena, Shelton, Max, Dalzell, Scott, Anderson, Bill, Harris-Shultz, Karen and Cremer, Julia (2013). Ecoturf - a case study on genetic variation and agronomic potential of bermudagrass (cynodon spp.) germplasm collected from Australian biodiversity. ITRC 2013: 12th International Turfgrass Research Conference, Beijing, China, 14-19 July 2013. Overland Park, KS, USA: Intertec Publishing.
O'Brien, C.M. and Drew, R.A. (2010). Marker-assisted hybridisation and backcrossing between Vasconcellea species and Carica papaya for prsv-p resistance. International Symposium on Molecular Markers in Horticulture, Corvallis, OR, United States, 29 July-1 August 2009. Leuven, Belgium: International Society for Horticultural Science. doi: 10.17660/actahortic.2010.859.43
Ashmore, S. E., Drew, R. A., O'Brien, C. and Parisi, A. (2009). Cryopreservation of Papaya (Carica papaya L.) Seed: Overcoming Dormancy and Optimizing Seed Desiccation and Storage Conditions. 1st International Symposium on Biotechnology of Fruit Species - BIOTECHFRUIT, Dresden, Germany, 14 July 2009. Leuven, Belgium: International Society of Horticultural Science.
Zhou, Y., Fukai, S., Cao, N., Ye, C. R., Le, T., Loch, D., Godwin, I., O'Brien, C., Dunne, R., Giles, H., Wilson, M., Halliday, M. and Lambrides, C. (2009). The use of infrared thermometer to detect drought resistance of green couch grass (Cynodon sp.) genotypes collected from the world'd driest inhabited continent. The 3rd International Conference on Integrated Approaches to Improve Crop Production Under Drought Prone Environments, Shanghai, China, 11-16 October 2009.
Drew, R., Siar, S. V., Dillon, S., Ramage, C., O'Brien, C. and Sajise, A. G. C. (2007). Intergeneric hybridisation between Carica papaya and wild Vasconcellea species and identification of a PRSV-P resistance gene. International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species, Daytona Beach, FL United States, 10-14 October 2005. Leuven, Belgium: International Society of Horticultural Science. doi: 10.17660/actahortic.2007.738.14
Dillon, S., O'Brien, C. M., Drew, R. A. and Ramage, C. (2006). Application of SCAR markers linked to a putative PRSV-P resistance locus for assisted breeding of resistant C-papaya cultivars. 5th International Symposium on In Vitro Culture and Horticultural Breeding, Debrecen, Hungary, 12-17 September 2004. Leuven, Belgium: International Society of Horticultural Science. doi: 10.17660/actahortic.2006.725.87
Azimi, M., O'Brien, C., Ashmore, S. and Drew, R. (2005). Cryopreservation of papaya germplasm. II International Symposium on Biotechnology of Tropical and Subtropical Species, Taipei, Taiwan, 5-9 November 2001. Leuven, Belgium: International Society for Horticultural Science. doi: 10.17660/actahortic.2005.692.4
Drew, R. A., Siar, S. V., Villegas, V. N., O´Brien, C. M. and Sajise, A. G. C. (2005). Development of PRSV-P resistant Carica papaya genotypes by introgression of genes from wild Vasconcella species. International Symposium on Harnessing the Potential of Horticulture in the Asian-Pacific Region, Coolum, QLD, Australia, 1-3 September 2004. Leuven, Belgium: International Society for Horticultural Science. doi: 10.17660/actahortic.2005.694.8
Drew, R., Persley, D., O'Brien, C. and Bateson, M. (2005). Papaya ringspot virus in Australia and the development of virus resistant plants. 2nd International Symposium on Biotechnology of Tropical and Subtropical Species, Taipei, Taiwan, 5-9 November 2001. Leuven, Belgium: International Society of Horticultural Science. doi: 10.17660/actahortic.2005.692.12
Drew, R.A., O'Brien, C.M. and Magdalita, P.M. (1998). Development of Carica interspecific hybrids. International Symposium on Biotechnology of Tropical and Subtropical Species, Brisbane, QLD Australia, 29 September - 3 October 1997. Leuven, Belgium: International Society of Horticultural Science. doi: 10.17660/actahortic.1998.461.31
Thesis
O'Brien, Christopher Michael (2021). Cryopreservation of avocado shoot tips for the conservation of Persea germplasm. PhD Thesis, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland. doi: 10.14264/7b83f15